| 摘要 |
<p>The present invention relates to a method for determining the ratio and quality of hybridomas by means of the number of differently labeled lysosomes, usually labeled with green and red fluorescence, where lysosomes originate from eukaryotic parent cells. Due to the property of lysosomes of fusing with one another, also yellow lysosomes formed by the fusion of green and red lysosomes may be found in the population of said green and red lysosomes. The physiological process of lysosomal fusion is one of the events involved in a complex system of expressing tumour and other antigens on the hybridoma surface. Therefore this new method gives, in addition to the data on the amount of hybridomas in a suspension of fused cells, at the same time also indirect information on the immunogenicity of hybridomas, which is of key importance for the effectiveness of a cellular vaccine against cancer in the paticnt's body.</p> |
