发明名称 |
METHODS FOR GENERATING HEPATOCYTES AND CHOLANGIOCYTES FROM PLURIPOTENT STEM CELLS |
摘要 |
Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates. |
申请公布号 |
SG11201506520T(A) |
申请公布日期 |
2015.09.29 |
申请号 |
SGT11201506520 |
申请日期 |
2014.02.18 |
申请人 |
UNIVERSITY HEALTH NETWORK;THE HOSPITAL FOR SICK CHILDREN |
发明人 |
KELLER, GORDON;OGAWA, SHINICHIRO;GHANEKAR, ANAND;BEAR, CHRISTINE;KAMATH, BINITA M.;OGAWA, MINA;SURAPISITCHAT, JAMES |
分类号 |
C12N5/071;A61K35/12;A61K35/407;A61P1/16;C12N5/0735;C12Q1/02 |
主分类号 |
C12N5/071 |
代理机构 |
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