发明名称 Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
摘要 A method for operating a nucleic acid sequencing instrument with movable flow cells. The method includes providing a flow cell having a flow path with an inlet port and an outlet port and filling the flow path, from the inlet port to the outlet port, with a first liquid reagent. The method also includes providing a station block having an inlet passage and an outlet passage, mounting the flow cell on the station block with the inlet port in fluid communication with the inlet passage and the outlet port in fluid communication with the outlet passage, introducing a gaseous bubble into the inlet port, conveying a second liquid reagent from the inlet passage into the inlet port to move the gaseous bubble through the flow path and into the outlet passage and fill the flow passage with the second liquid reagent, and removing the flow cell from the station block.
申请公布号 US9146248(B2) 申请公布日期 2015.09.29
申请号 US201313802957 申请日期 2013.03.14
申请人 Intelligent Bio-Systems, Inc. 发明人 Hagerott Thomas;Golaski Edmund W.
分类号 G01N35/10;B01L3/00 主分类号 G01N35/10
代理机构 RatnerPrestia 代理人 RatnerPrestia
主权项 1. A method for operating a nucleic acid sequencing instrument having one or more movable flow cells, the method comprising: providing a flow cell comprising a flow path having an inlet port and an outlet port; filling the flow path, from the inlet port to the outlet port, with a first liquid reagent; providing a station block having an inlet passage and an outlet passage; mounting the flow cell on the station block to place the inlet port in fluid communication with the inlet passage and the outlet port in fluid communication with the outlet passage; introducing a gaseous bubble into the inlet port by: evaporating a portion of a second liquid reagent located in the inlet passage to create a gas space at a top end of the inlet passage prior to mounting the flow cell on the station block, andcapturing gas in the gas space upon mounting the flow cell to the station block to thereby form the gaseous bubble; conveying a second liquid reagent from the inlet passage into the inlet port to move the gaseous bubble through the flow path and into the outlet passage and fill the flow passage with the second liquid reagent; and removing the flow cell from the station block.
地址 Waltham MA US