Thread-based microfluidic guiding system

摘要

A thread-based microfluidic guiding system is provided and includes a substrate, two fiber threads arranged on the substrate in a cross manner. The two fiber threads are used as physical guiding pathways to guide a sample fluid and a buffer fluid, respectively. The two fiber threads have capillary action, so that the sample fluid, the buffer fluid or a mixture fluid thereof can flow along fiber surfaces of the two fiber threads, which can be pre-treated by plasma. The thread-based microfluidic guiding system of the present invention is different from recessed microfluidic channel structures, and can simplify system structure, lower manufacture cost, accelerate detection operation and enhance detection sensitivity.

主权项

1. A thread-based microfluidic guiding system, comprising:
a substrate; a first fiber thread disposed on the substrate and sequentially having a sample fluid injection end, a sample fluid transmission section, a first turning portion, a mixture fluid transmission section, and a first waste fluid end, wherein the sample fluid transmission section is turned and coupled to the mixture fluid transmission section through the first turning portion; a second fiber thread disposed on the substrate and sequentially having a buffer fluid injection end, a buffer fluid transmission section, a second turning portion, a waste fluid transmission section, and a second waste fluid end, wherein the buffer fluid transmission section is turned and coupled to the waste fluid transmission section through the second turning portion, and the first turning portion crosses and contacts the second turning portion; a working electrode disposed on the substrate and contacting the mixture fluid transmission section to form a detection area which is close to the first waste fluid end; a counter electrode which is disposed on the substrate and in contact with the mixture fluid transmission section, close to the working electrode, and located between the working electrode and the first waste fluid end; a reference electrode which is disposed on the substrate and in contact with the mixture fluid transmission section, and located between the working electrode and the counter electrode; and a decoupler electrode which is disposed on the substrate and in contact with the mixture fluid transmission section, close to the working electrode, and located between the first turning portion and working electrode; wherein each of the working electrode, the counter electrode, the reference electrode and the decoupler electrode has a flat top surface parallel to a top surface of the substrate, and a groove configured as a depression in the flat top surface and having a plurality of interior wall surfaces for contacting with the mixture fluid transmission section.