Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides

摘要

Compositions and methods are provided for enriching mitochondrial DNA and optionally chloroplast DNA from eukaryotic cells in a simple rapid method that provides greater than 100 fold enrichment. Affinity protein-coated substrate in a buffer is used to efficiently bind chromosomal DNA and thereby remove it from the buffer. Mitochondrial sequencing reads reveal that non-biased sequence selection providing representation of a substantial proportion of mitochondrial DNA in the eukaryotic cells analyzed.

主权项

1. A composition comprising:
a mixture comprising eukaryotic chromosomal DNA and mitochondrial DNA, wherein the chromosomal DNA in the mixture has a median size of at least 10 kb, a matrix comprising a protein that comprises a methyl binding domain, wherein the protein is capable of selectively binding to methylated polynucleotides but not non-methylated polynucleotides, and a buffer containing effective amounts of a salt and a non-ionic detergent.