Process for culturing adherent cells

摘要

The subject of the present invention is a process for producing adherent cells comprising:
a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium;b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out:
i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, andii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; andc. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers.;The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.

主权项

1. A process for producing adherent cells comprising:
a) introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium, wherein the culture vessel is in direct physical contact with the culture medium; b) amplifying the cells by performing a plurality of cell passages in the same culture vessel, wherein each cell passage subsequent to the first cell passage is carried out:
i) by retaining in the culture vessel all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment in the culture vessel to detach the cells from microcarriers without removing the all or part of the cells produced during the previous cell passage from the culture vessel, andii) by introducing culture medium and an increasing amount of microcarriers into the culture vessel; and c) harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers.