摘要 |
<p>The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings such as poly(N-(5 azidoacetamidylpentyl)acrylamide co acrylamide) (PAZAM) in the formation and manipulation of substrates such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer such as PAZAM and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.</p> |