发明名称 |
A PHARMACEUTICAL COMPOSITION FOR STIMULATION OF ANGIOGENESIS. |
摘要 |
Provided is a pharmaceutical composition for growth induction in blood vessel tissue which contains purified plasmid DNA encoding a vascular endothelial growth factor (VEGF) and pharmaceutically acceptable excipients that include a cryoprotectant as a vehicle and/or a pH stabilizer for stabilizing the pH in the range of 7.0 to 9.0, in effective quantities. Also provided is a storage method of plasmid DNA which encodes a VEGF comprising mixing the purified plasmid DNA with a solution of at least one cryoprotectant having properties of a vehicle and/or a pH stabilizer in the pH range of 7.0-9.0. The solution is lyophilized and stored at +2 to +8ºC. Supercoiled DNA pCMV-VEGF165 may be used which is produced by culturing Esherichia coli strain TOP10/pCMV-VEGF165. The pharmaceutical composition is administered to a human in quantities sufficient to provide a necessary therapeutic effect. The provided pharmaceutical composition of plasmid DNA pCMV-VEGF165 does not change significantly properties of the active substance when stored for a long time at a temperature from +2 to +8ºC. |
申请公布号 |
MX2015002658(A) |
申请公布日期 |
2015.09.25 |
申请号 |
MX20150002658 |
申请日期 |
2013.08.02 |
申请人 |
"NEXTGEN" COMPANY LIMITED |
发明人 |
SERGEJ L' VOVICH KISELEV;ROMAN VADIMOVICH DEEV;IVAN IVANOVICH VOROB´EV;NADEZHDA ALEKSANDROVNA ORLOVA;ARTHUR ALEKSANDROVICH ISAEV |
分类号 |
C12N1/21;A61K35/74;A61K38/18;A61P9/00;C07K14/435;C07K14/475;C12N15/09;C12N15/10;C12N15/12;C12N15/63;C12R1/19 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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