METHOD FOR IMPARTING ACID RESISTANCE AND SALT RESISTANCE, AND USEFUL SUBSTANCE PRODUCTION USING ACID-RESISTANT, SALT-RESISTANT YEAST

摘要

Provided are: a method for preparing yeast presenting various types of growth inhibition resistance and fermentation inhibition resistance in strong-acid (low-pH) and high-salt-concentration environments by introducing genes and proteins presenting resistance to acid and salt derived from acid-resistant, salt-resistant yeast into yeast having exceptional fermentation production or by improving the expression level of such attributes, whereby acid resistance and salt resistance are imparted; and a method for producing useful substances such as ethanol by using this method. Genetically modified yeast constantly expressing an LGS1 gene derived from Issatchenkia orientalis or an LGS1 homologous gene (GAS1, PHR1, or PHR2 gene, or the like) belonging to the GH72 family and derived from another yeast, a method for achieving resistance in low-pH and high-salt-concentration environments using the same, and a method for highly efficiently producing useful substances such as ethanol and the like from glucose or a saccharified solution containing glucose.