| 摘要 |
The invention provides compositions and methods for preserving a biological material—such as a protein, a nucleic acid or a biological sample, or any combination thereof—in a substantially water-free, nonionic or ionic organic solvent. Improved preservation, including for example the stability and/or the solubility of the biological material in the substantially water-free fluid medium, is achieved with compositions comprising one or more substances (e.g., an antioxidant) described in the disclosure, and/or a metal salt. The biological material is soluble and stable, and retains its function and activity, when it is preserved in the substantially water-free fluid medium at ambient temperature or higher for extended periods of time. Therefore, the composition comprising the biological material does not need to be refrigerated or frozen during shipping or storage. |
| 主权项 |
1. A composition comprising:
a) biological material, comprising a polypeptide, a polynucleotide or a biological sample, or any combination thereof, and b) at least one solvent, wherein said solvent or solvents are
(i) at least one alcohol solvent selected from the group consisting of linear and branched C2-C6 acyclic alcohols having one or more hydroxyl groups and C3-C6 cyclic alcohols having one or more hydroxyl groups and three to six ring carbon atoms, wherein the acyclic alcohols and the cyclic alcohols optionally comprise one or more halide atoms; or(ii) at least one ionic organic solvent comprising an organic salt and an organic hydrogen bond donor,wherein the composition is in a fluid state and is substantially free of water. |
