| 摘要 |
An immunochromatography for multi-item detection is provided which contains detecting and measuring fluorescence and light absorption respectively at once with a detection device. The immunochromatography contains using fluorescent particles and light absorbing particles, wherein the fluorescence excitation wavelength of the fluorescent particles and the absorption wavelength of the light absorbing particles are in the same wavelength region; and detecting and measuring, at once, the intensity of reflected light from a test area, the intensity of reflected light from another test area, and the intensity of reflected light from a non-test area other than the test areas. |
| 主权项 |
1. An immunochromatography for multi-item detection, comprising steps of:
applying, to a test piece having fluorescent particles and light absorbing particles as labeling particles, a liquid sample suspected of containing target substances A and B, so that the fluorescent particles and the light absorbing particles are allowed to flow; and detecting and measuring fluorescence and light absorption respectively at once with a detection device, wherein the fluorescence is derived from the fluorescent particles captured in a test area of the test piece, and the light absorption is derived from the light absorbing particles captured in another test area of the test piece, wherein the detection device comprises a detection unit for detecting fluorescence and light absorption, and a control unit for controlling the detection unit, wherein the fluorescent particles and the light absorbing particles are such that the fluorescent particles have a fluorescence excitation wavelength (λ1), the light absorbing particles have an absorption wavelength (λ2), the fluorescence excitation wavelength (λ1) and the absorption wavelength (λ2) are in the same wavelength region, the target substance A is captured by the fluorescent particles when detected, the target substance B is captured by the light absorbing particles when detected, and the target substance A and the target substance B are different from each other, wherein the test area nt1 where the fluorescent particles are captured and the test area nt2 where the light absorbing particles are captured each are provided with the test piece at different positions, and wherein the control unit of the detection device commands the detection unit to scan the test piece and to detect and measure, at once, the intensity of reflected light from the test area nt1, the intensity of reflected light from the test area nt2, and the intensity of reflected light from a non-test area other than the test areas nt1 and nt2. |
