HPPD VARIANTS AND METHODS OF USE

摘要

Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

主权项

1. A recombinant nucleic acid molecule encoding a 4-hydroxyphenylpyruvate dioxygenase (HPPD) enzyme, wherein said nucleic acid molecule encodes an amino acid sequence having at least 55% sequence identity to SEQ ID NO:1, or a fragment thereof, and has one or more amino acid substitutions selected from the group consisting of:
(a) a tryptophan, glycine, or serine at the amino acid position corresponding to amino acid position 188 of SEQ ID NO:1; (b) a serine, cysteine, or arginine at the amino acid position corresponding to amino acid position 189 of SEQ ID NO:1; (c) a proline, serine, histidine, alanine, glycine, or glutamine at the amino acid position corresponding to amino acid position 335 of SEQ ID NO:1; (d) a serine or tryptophan at the amino acid position corresponding to amino acid position 336 of SEQ ID NO:1; (e) a threonine, alanine, or serine at the amino acid position corresponding to amino acid position 339 of SEQ ID NO:1; (f) a glutamine, alanine, valine, or glutamic acid at the amino acid position corresponding to amino acid position 340 of SEQ ID NO:1; or (g) a leucine at the amino acid position corresponding to amino acid position 215 of SEQ ID NO:1;wherein said HPPD enzyme is tolerant to an HPPD inhibitor herbicide.