CULTURE MEDIUM ADDITIVE FOR USE IN SERUM-FREE CULTURING OF ANIMAL CELL, KIT AND USE THEREOF

摘要

Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

主权项

1. A method for serum-free culturing of a mesenchymal stem cell or a monkey kidney-derived undifferentiated cell line COS7 cell, the method comprising the step of:
culturing a mesenchymal stem cell or a monkey kidney-derived undifferentiated cell line COS7 cell in a culture medium containing:
at least three growth factors selected from the group consisting of FGF, PDGF, TGF-β, and HGF; andat least one phospholipid.