METHOD OF SELECTING A MONOCLONAL CELL COLONY

摘要

A method of selecting a monoclonal cell colony that secretes a product of interest providing a plurality of cell culture spaces; each cell culture space has a respective assessment surface with a first binding agent immobilised on the assessment surface. The cells are incubated in a growth medium in the cell culture spaces for replication of the cells. At least some of the cells secrete a product of interest. A second binding agent is provided in the cell culture spaces. Initially, the second binding agent is not immobilised. Each of the first and second binding agents binds to the product of interest so that secretion of the product of interest by cells in any one of the cell culture spaces leads to a complex with the first binding agent, the product of interest and the second binding agent forming at the corresponding assessment surface of the cell culture space.

主权项

1. A method of selecting a monoclonal cell colony that secretes a product of interest, comprising:
providing a plurality of cell culture spaces, each cell culture space having a respective assessment surface; providing each said assessment surface with a first binding agent that is immobilised on the assessment surface; plating a suspension of cells in the plurality of cell culture spaces so that at least some of the cell culture spaces are plated with a single viable cell; incubating the cells in a growth medium in the cell culture spaces to cause replication of the cells, wherein at least some of the cells secrete a product of interest; providing a second binding agent in the cell culture spaces, the second binding agent not being immobilised; wherein each of the first and second binding agents binds to the product of interest so that secretion of the product of interest by cells in any one of the cell culture spaces leads to a complex comprising the first binding agent, the product of interest and the second binding agent forming at the corresponding assessment surface of the said one cell culture space; assessing each of at least some of the cell culture spaces for presence of the second binding agent at the assessment surface of the cell culture space; determining for each of at least some of the cell culture spaces whether the cell culture space contains a single cell colony that is derived from a single cell; and selecting a cell culture space using said assessment and said determination, the selected cell culture space containing a single monoclonal cell colony which secretes the product of interest.