Zwitterionic stationary phase for hydrophilic interaction liquid chromatography and preparation method thereof

摘要

A type of liquid chromatographic stationary phase and preparation method thereof, the bonding terminal of the chromatographic stationary phase is zwitterionic functional group. The preparation method includes the following steps, alkenyl or alkynyl silane is bonded onto the surface of silica based on the horizontal polymerization approach to obtain alkenyl- or alkynyl-modified silica. Then the thiol click reaction with zwitterionic compound containing thiol group is performed to obtain the zwitterionic hydrophilic interaction chromatographic stationary phase. The present stationary phase possesses both zwitterionic characteristics and excellent hydrophilicity. It can be widely applied in the separation of variety of samples.

主权项

1. A zwitterionic hydrophilic interaction chromatographic stationary phase, comprising:
a silica substrate; a bonded phase affixed to the silica substrate, wherein the bonded phase comprises zwitterionic functional groups  and —(CH2)n-R2 groups affixed to the silica substrate; wherein  is an amino acid, a peptide, or a protein, X+ represents a positively charged amino group, Y− represents a negatively charged carboxyl group, wherein R2 is a vinyl group or an acetenyl group, n is 0 to 4, wherein the stationary phase is schematically represented as follows: