Analytical sandwich test for determining NT-proBNP

摘要

The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. The epitopes recognized by the antibodies can slightly overlap.

主权项

1. An immunological test strip device for determining human N-terminal pro-brain natriuretic peptide (NT-proBNP) comprising:
(a) a sample application zone for receiving a liquid sample; (b) a combination of antibodies, wherein the combination is:
i. a monoclonal antibody that specifically binds an epitope of NT-proBNP, wherein the epitope comprises amino acid residues 27-31 of the sequence of human NT-proBNP;ii. a polyclonal antibody that specifically binds an epitope of NT-proBNP, wherein the epitope is amino acid residues 38-42 of the sequence of human NT-proBNP;wherein at least one antibody in the combination of antibodies is attached to a particulate label selected from the group consisting of a gold label, a colored lattice, a metal sol label, a polymer label, a semiconductor nanocrystal and biotin; and (c) a detection zone in fluid communication with the sample application zone for binding a complex comprising the NT-proBNP and the antibodies of the combination.