Assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity

摘要

Methods of conducting an assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity are provided. The methods include concentrating the glycoprotein having enzymatic activity by capturing the glycoproteins from the input sample on a solid support, washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity, and measuring enzymatic modification of the substrate. In some examples, the capturing of the glycoprotein and subsequent washing steps are performed in one or more droplets in oil.

主权项

1. A method of conducting an assay for measuring enzymatic modification of the substrate by a glycoprotein having enzymatic activity, the method comprising:
(a) preparing an output sample comprising glycoproteins having enzymatic activity, comprising:
(i) providing an input sample comprising glycoproteins having enzymatic activity;(ii) capturing the glycoproteins from the input sample on a solid support;(iii) washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, wherein steps (ii) and (iii) are performed in one or more droplets in oil; and(iv) eluting glycoproteins from the solid support to yield an output sample comprising glycoproteins having enzymatic activity; (b) adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity; and (c) measuring enzymatic modification of the substrate.