| 主权项 |
1. A method of detecting the presence of a nucleic acid polymer in a sample comprising:
a) contacting a sample suspected of containing a nucleic acid polymer with a composition comprising a conjugate comprising a compound of Formula (I) and an oligonucleotide; and b) detecting the presence or amount of the compound in the sample; wherein the compound of formula (I) is a component in an ET cassette; and wherein the compound of formula (I) is as shown below:wherein
Z is Si(R11)(R12) or Ge(R11)(R12); each R11 and R12 are independently selected from C1-10 linear, branched or cyclic alkyl, C1-10 alkyl interrupted with one or more heteroatoms, C6-10 aryl, heteroaryl or R11 and R12 may together form a ring; each X is independently selected from OR2 or N(R3)(R4); each R1 is independently selected from H, C1-4 alkyl, sulfonate or halo; R2 is H, C1-10 alkyl, L-R or L-CS; R3 and R4 are independently selected from H, C1-4 alkyl, C1-4 alkyl interrupted with one or more heteroatoms, C6-10 aryl, peptidyl, heteroaryl, L-R or L-CS; R6-10 are independently H, halo, alkoxy, amino, alkyl, aryl, heteroaryl, CO2H, SO3H, L-CO2H, L-SO3H, L-R or L-CS; L is a covalent linkage that is linear or branched, cyclic or heterocyclic saturated or unsaturated, having 1-16 non hydrogen atoms such that the linkage contains any combination of ester, acid, amine, amide, alcohol, ether, thioether or halide groups or single, double, triple or aromatic carbon-carbon bond; R is a reactive group; or CS is a conjugated substance; one or more of R3 and R4 or R3 and R1 or R4 and R1 may together form a ring;
and one or more of R6-10 may together form a ring. |
