Method for detection of microorganism and kit for detection of microorganism

摘要

Live cells of a microorganism in a test sample are detected by the following steps:
a) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison,b) the step of extracting DNA from the test sample, and amplifying a target region of the extracted DNA by PCR, andc) the step of analyzing an amplification product.

主权项

1. A method for detecting live cells by distinguishing the live cells from dead cells and also from injured cells of microorganisms in a test sample, which comprises the following steps:
a) treating the test sample with two poisons, wherein a first poison is a topoisomerase poison and a second poison is a DNA gyrase poison, b) extracting DNA from the test sample and amplifying a target region of the extracted DNA by PCR, wherein the length of the target region is 100 to 3000 nucleotides, c) analyzing a resulting PCR amplification product, and d) distinguishing the live cells from the dead cells and also from injured cells, wherein the method does not comprise treatment with ethidium monoazide.