发明名称 |
Polypeptide targeting to mitochondria |
摘要 |
Methods for delivering non-mitochondrial proteins to mitochondria are provided. Also provided are nucleic acid constructs comprising a coding sequence encoding a DNA-binding polypeptide, fused to a mitochondrial targeting sequence (MTS) and a nuclear export signal (NES), and the encoded proteins. The construct successfully delivers DNA binding proteins to the mitochondrion. A chimeric methylase based on the above construct is successfully delivered to mitochondria, resulting in modification of mtDNA. |
申请公布号 |
US9139628(B2) |
申请公布日期 |
2015.09.22 |
申请号 |
US200812143886 |
申请日期 |
2008.06.23 |
申请人 |
Medical Research Council |
发明人 |
Minczuk Michal;Papworth Monika A.;Klug Aaron |
分类号 |
C12N15/63;A61K38/00;C07K14/47 |
主分类号 |
C12N15/63 |
代理机构 |
Veddar Price P.C. |
代理人 |
Veddar Price P.C. ;Kowalski Thomas J.;Lu Deborah L. |
主权项 |
1. A nucleic acid construct for the modulation of gene expression in a mitochondrion of a eukaryotic cell, said construct comprising a coding sequence encoding a zinc finger DNA-binding polypeptide comprising at least 4 zinc fingers, fused at the N-terminus to a mitochondrial targeting sequence (MTS) and at the C-terminus to a nuclear export signal (NES), wherein the zinc finger DNA-binding polypeptide is biased towards nuclear localization and use of the MTS alone results in a predominantly nuclear localization, and wherein the zinc finger DNA-binding polypeptide is heterologous to mitochondria. |
地址 |
London GB |