发明名称 |
Ketoreductase polypeptides |
摘要 |
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe. |
申请公布号 |
US9139820(B2) |
申请公布日期 |
2015.09.22 |
申请号 |
US201514606127 |
申请日期 |
2015.01.27 |
申请人 |
Codexis, Inc. |
发明人 |
Mundorff Emily;De Vries Erik Jan |
分类号 |
C12N9/00;C12P7/02;C12N9/04;C12P17/14;C12P17/10 |
主分类号 |
C12N9/00 |
代理机构 |
Codexis, Inc. |
代理人 |
Codexis, Inc. |
主权项 |
1. An engineered polypeptide having ketoreductase activity, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 161. |
地址 |
Redwood City CA US |