Fremgangsmåde til fremstilling af glykosidase, enzymsammensætning og biomassehydrolyseringsfremgangsmåde

摘要

A glucosidase having improved cellulose degradation efficiency is provided and an efficient method for hydrolyzing biomass using the glucosidase is provided. The present invention provides a method for producing a mutant glucosidase, which includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. The present invention also provides an enzyme composition containing the mutant glucosidase and a method for hydrolyzing biomass using the enzyme composition.