METHODS AND SYSTEMS FOR A DIGITAL PCR EXPERIMENT DESIGNER

摘要

A computer- implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving , from a user , a selection of optimization type. The optimization type may be maximizing the dynamic range , minimizing the number of substrates including reaction sites needed for the experiment , determining a dilution factor , or determining the lower limit of detection , for example. The method further includes receiving , from the user , a precision measure for an experiment , and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.