NOVEL PRONGF MUTANTS AND USES THEREOF IN THE PRODUCTION OF BETA-NGF PRONGF -NGF
摘要
The present invention relates to a proNGF mutant and to uses thereof, in particular the use of a proNGF mutant for producing human beta-NGF. The present invention discloses a method of preparing a biologically active human beta-NGF from an inactive insoluble proNGF mutant. A proNGF mutant of the invention is substituted by any amino acid but not Arg or Lys at the native protease cleavage site R 1 SK 3 R 4 at least at positions R 1 and K 3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence.
申请公布号
HK1202055(A1)
申请公布日期
2015.09.18
申请号
HK20150102539
申请日期
2015.03.12
申请人
WACKER CHEMIE AG
发明人
LOREY, SUSAN S;JANOWSKI, BERNHARD B;PULTKE, HEIKO H;KATHMANN, DANIELA D;PARTHIER, ANTJE A;ANTON, ANDREAS A