发明名称 Method for Producing Basic Substance
摘要 A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
申请公布号 US2015259716(A1) 申请公布日期 2015.09.17
申请号 US201414483588 申请日期 2014.09.11
申请人 AJINOMOTO CO., INC. 发明人 Takeshita Ryo;Sugimoto Shinichi
分类号 C12P13/10;C12P13/08 主分类号 C12P13/10
代理机构 代理人
主权项 1. A method for producing a basic amino acid by fermentation comprising culturing a microorganism having an ability to produce the basic amino acid in a liquid medium contained in a fermentation tank to produce and accumulate the basic amino acid in the medium; wherein the amount of sulfate and/or chloride ions used as counter ions of the basic amino acid is reduced by adjusting the total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of the culture process including a period where the pH of the medium increases due to shortage of the counter ions caused by accumulation of the objective basic amino acid; wherein the total ammonia concentration in the medium is adjusted by adding ammonia or urea to the medium when the activity of the microorganism is reduced or ceases as determined based on the indicators: dissolved oxygen concentration in the medium, consumption rate of carbon source in the medium, turbidity of the medium, productivity of the basic amino acid, and pH change in the medium; and wherein the microorganism is an Escherichia coli bacterium or a coryneform bacterium.
地址 Tokyo JP