CALIBRATORS FOR USE IN HIGH-RESOLUTION MELT ANALYSIS AND RELATED METHODS

摘要

Calibrators for use in high-resolution melt analysis and related methods are disclosed and described. In one embodiment a polynucleotide thermal melt calibrator having a stem-loop configuration may include: a first stem region having a first nucleotide sequence; a second stem region having a second nucleotide sequence; a loop region joining the first stem region and the second stem region; and a hybridization indicator associated with at least one of the first stem region, the second stem region, or the loop region.

主权项

1. A polynucleotide thermal melt calibrator having a stem-loop configuration, comprising:
a first stem region having a first nucleotide sequence; a second stem region having a second nucleotide sequence, wherein the second nucleotide sequence is sufficiently complimentary to the first nucleotide sequence to at least substantially hybridize and at least substantially denature across a temperature range of about 10° C. or less; a loop region joining the first stem region and the second stem region, the loop region having a loop sequence and a loop length to allow hybridization of the first stem region and the second stem region; a hybridization indicator associated with at least one of the first stem region, the second stem region, or the loop region.