METHODS OF PRODUCING ACETOIN AND 2,3-BUTANEDIOL USING PHOTOSYNTHETIC MICROORGANISMS

摘要

The present disclosure provides recombinant cyanobacteria having acetolactate synthase activity and acetolactate decarboxylase activity, as well as recombinant cyanobacteria having acetolactate synthase activity, acetolactate decarboxylase activity, and secondary alcohol dehydrogenase activity. Methods for the production of the recombinant cyanobacteria, as well as methods for use thereof for production of acetoin and 2,3-butanediol from carbon dioxide and light are also provided.

主权项

1. A cyanobacterium comprising a recombinant polynucleotide encoding an acetolactate synthase (ALS) and an acetolactate decarboxylase (ALDC), wherein the ALS is a bacterial ALS and the ALDC is a bacterial ALDC or a fungal ALDC, and wherein expression of the ALS and the ALDC results in an increase in production of acetoin as compared to a corresponding cyanobacterium lacking the polynucleotide.