| 主权项 |
1. A method for constructing a separation device to separate a solute in a fluid sample, said method comprising:
a) preparing a gellable polymer in a liquid state; b) adding a predetermined volume of said gellable polymer in the liquid state into a cylindrical porous support having a lumen, a main axis, an inside diameter, an outside diameter, an inside wall, and a bottom end, said gellable polymer being added into said lumen, wherein said main axis of said cylindrical porous support is vertically oriented, and said bottom end is sealed; c) placing a cylindrical rod into said lumen of said cylindrical porous support concentrically with said main axis, said cylindrical rod having a diameter smaller than said inside diameter of said cylindrical porous support, forming a substantially uniform gap between said cylindrical rod and said inside wall, whereby a substantially uniform fluid gellable polymer layer is formed in said substantially uniform gap, and wherein said predetermined volume is sufficient to fill said gap; d) inducing said gellable polymer to gel, whereby a porous separation matrix of substantially uniform thickness is formed; e) upwardly lifting said cylindrical rod from said cylindrical porous support; and, f) encasing said porous separation matrix and said inert cylindrical porous support in a non-porous housing, said non-porous housing including a fluid inlet and a fluid outlet, said nonporous housing, fluid inlet and fluid outlet configured to deliver and constrain said fluid sample to flow through pores of said porous separation matrix and to collect purified solutes from said separation device. |
