发明名称 |
DIP-STICK WESTERN BLOT |
摘要 |
Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick. |
申请公布号 |
US2015260681(A1) |
申请公布日期 |
2015.09.17 |
申请号 |
US201514644527 |
申请日期 |
2015.03.11 |
申请人 |
BIO-RAD LABORATORIES, INC. |
发明人 |
Strong William |
分类号 |
G01N27/447;G01N33/543 |
主分类号 |
G01N27/447 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method of separating and detecting analytes of a sample, the method comprising:
applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick, wherein separating analytes of the sample comprises performing electrophoresis, electroosmosis, or isoelectric focusing. |
地址 |
Hercules CA US |