发明名称 |
Methods for Isolating and Proliferating Autologous Cancer AntiGen-Specific CD8+ T Cells |
摘要 |
Provided is a method for isolating and proliferating autologous cancer antigen-specific CD8+T cells, and more particularly, a method for selecting an epitope recognized by CD8+ T cells from autologous cancer antigens present in blood of individual cancer patients; and isolating autologous cancer antigen-specific CD8+ T cells by using a peptide of the selected epitope, and a method of massively proliferating CD8+ T cells by using the method. According to the present invention, it is possible to isolate autologous cancer antigen-specific CD8+ T cells by using the peptide of the CD8 T cell epitope of the autologous cancer antigen present in blood of individual cancer patients instead of a heterologous antigen. Therefore, by using T cells recognizing the autologous cancer antigen, it is possible to effectively select and eliminate cancer cells derived from the cancer patient's own cells. Thus, T cells can be applied to treatment and alleviation of cancer diseases without side effects. |
申请公布号 |
US2015259646(A1) |
申请公布日期 |
2015.09.17 |
申请号 |
US201514656355 |
申请日期 |
2015.03.12 |
申请人 |
National Cancer Center |
发明人 |
Kwon Byoung Se;Kang Hyun-Guy;Kim Kwang-Hui;Kim Young-Woo;Kim Young-Ho;Park Byung-Kiu;Park Sang-Yoon;Park Sang-Jae;Eom Hyeon-Seok;Oh Ho-Sik;Yoo Heon;Lee Don-Gil;Lee Seung-Hoon;Lee Young-Joo;Lee Jin-Soo;Choi Beom-Kyu |
分类号 |
C12N5/0783 |
主分类号 |
C12N5/0783 |
代理机构 |
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代理人 |
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主权项 |
1. A method for isolating autologous cancer antigen-specific CD8+ T cells, the method comprising:
a) selecting a CD8+ T cell epitope of an autologous cancer antigen present in blood of a cancer patient; b) culturing a peripheral blood mononuclear cell (PBMC) isolated from blood of the cancer patient in a medium together with a peptide of the epitope and IL-2; c) inducing 4-1BB expression in the cultured cells by adding the peptide same as in step b); and d) culturing cells in which 4-1BB expression is induced on a culture plate coated with an anti-4-IBB antibody, and then removing unattached cells. |
地址 |
Gyeonggi-do KR |