ARRAY OF MICROMOLDED STRUCTURES FOR SORTING ADHERENT CELLS

摘要

An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

主权项

1. An apparatus for collecting or culturing cells or cell colonies, said apparatus comprising:
a substrate formed from an elastomer and having a first surface and an opposed second surface and a plurality of wells formed in the first surface in the form of an array, and a plurality of rigid cell carriers each carrier disposed in one of said wells, the carriers configured to release from said substrate upon mechanical distortion of said substrate.