| 摘要 |
A method of mass spectrometry or ion mobility spectrometry comprises providing a spectrometer comprising an orifice between an atmospheric pressure region and a sub-atmospheric pressure region of the spectrometer, wherein the sub-atmospheric pressure region comprises an ion guide or ion trap: providing a sample probe comprising a needle assembly on which a sample is deposited or that is supplied with a sample; inserting the needle assembly through the orifice and into the sub-atmospheric pressure region so that the sample is arranged within or adjacent to the ion guide or ion trap in the sub-atmospheric pressure region; and then desorbing the sample from the needle assembly within the sub-atmospheric pressure region and/or ionising the sample within the sub-atmospheric pressure region so as to generate ions that enter the ion guide or ion trap. Also disclosed is the mass or ion mobility spectrometer used in the above method. Further disclosed is a method of mass or ion mobility spectrometry as above, where the needle assembly has a solid phase micro-extraction (SPME) device holding a sample in the solid phase and where a laser is directed onto the sample to ionise it. As the needle assembly is inserted so that the sample is arranged within or adjacent to the ion guide or ion trap, analyte ions from the sample are captured efficiently. |
