发明名称 FOREIGN SUBSTANCE INTRODUCTION DEVICE AND METHOD OF PRODUCING CELLS WITH INTRODUCED FOREIGN SUBSTANCE
摘要 An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells efficiently and at low cost by electrical action and a method for manufacturing an exogenous substance-bearing cell that is able to manufacture many kinds of exogenous substance-bearing cells at low cost are provided. In the introducing of an exogenous substance such as a gene or the like into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container (2) through an opening portion (17). The cell suspension liquid is supplied to the container (2) in an amount that is prepared such that a liquid droplet (W) that is formed will not come into contact with a pair of electrodes (5a, 5b) at the same time. The liquid droplet (W) is formed in an oil (6) that is stored in the container (2), without the cell suspension liquid mixing with the oil (6). The liquid droplet (W) is disposed between the electrodes (5a, 5b). When a direct current voltage is supplied to the electrodes (5a, 5b) from a power supply (9), the liquid droplet (W) moves between the electrodes (5a, 5b), and the exogenous substance is introduced into the cell within the liquid droplet (W) by electrical action when the liquid droplet (W) comes into contact with the electrodes (5a, 5b).
申请公布号 EP2918667(A1) 申请公布日期 2015.09.16
申请号 EP20130861819 申请日期 2013.12.12
申请人 NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY 发明人 MIZUNO, AKIRA;NUMANO, RIKA;KURITA, HIROFUMI
分类号 C12M1/00;C12M1/42;C12N1/00;C12N5/00;C12N5/10;C12N15/87 主分类号 C12M1/00
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