| 摘要 |
The invention relates to an antibody or an antigen-binding fragment thereof which specifically binds the ±3 domain of a HLA-G protein, in particular binds the ²2-microglobulin free HLA-G protein exhibiting an ±3 domain. The invention also relates to nucleic acid molecules encoding a human HLA-G ±3 domain polypeptide, which is selected from a group of specific sequences, as well as vectors for the cloning and/or for the expression such nucleic acid molecules, recombined cells or cell lines and compositions for use in a host in need thereof to interfer with and in particular to neutralize the immune down-regulation due to HLA-G proteins, and/or improving or treating conditions showing HLA-G+ lesions, and/or improving or treating a neoplasic condition or disease, in particular a cancer disease. The invention also relates to a method of production of an antibody or an antigen-binding fragment thereof of the invention, and to immunogenic compositions or means of the invention for use to elicit in a host an immune response against the ±3 domain of HLA-G protein. The invention also relates to an in vitro method for detecting HLA-G protein in a sample and/or monitoring or diagnosing a health status or pathologic condition through the analysis of a sample and kit for such an in vitro assay. |
