High throughput multiwell system for culturing 3D tissue constructs in-vitro or in-vivo, method for producing said multiwell system and methods for preparing 3D tissue constructs from cells using said multiwell system

摘要

The invention relates to a multiwell system, characterized that said multiwell system having at least 3 wells, wherein said wells have a volume between 0.125 and 4.0 mm3.

主权项

1. A method for preparing a 3D tissue construct from cells comprising the steps of:
a) introducing a medium comprising cells in a well of a multiwell system,
wherein the multiwell system comprises at least 3 wells, the wells comprises sidewalls made of alginate, polylactic acid or PEOT/PBT, wherein said wells have a volume between 0.125 and 4.0 mm3, a wall thickness of at least 50 microns and a closed bottom, wherein the multiwell system does not comprise a 3D synthetic polymer scaffold and the initial number of cells per well is between 4,000 and 25,000; and b) culturing the cells to obtain a 3D tissue construct.