发明名称 |
ENZYME-CATALYZED METAL DEPOSITION FOR THE ENHANCED IN SITU DETECTION OF IMMUNOHISTOCHEMICAL EPITOPES AND NUCLEIC ACID SEQUENCES |
摘要 |
<p>The invention is directed to novel compositions of matter and methods of detecting in situ an immunohistochemical epitope or nucleic acid sequence of interest in a biological sample comprising binding an enzyme-labeled conjugate molecule to the epitope or sequence of interest in the presence of a redox-inactive reductive species and a soluble metal ion, thereby facilitating the reduction of the metal ion to a metal atom at or about the point where the enzyme is anchored. Novel phosphate derivatives of reducing agents are described that when exposed to a phosphatase are activated to their reducing form, thereby reducing metal ions to insoluble metal.</p> |
申请公布号 |
CA2773566(C) |
申请公布日期 |
2015.09.15 |
申请号 |
CA20042773566 |
申请日期 |
2004.06.24 |
申请人 |
VENTANA MEDICAL SYSTEMS, INC. |
发明人 |
BIENIARZ, CHRISTOPHER;KERNAG, CASEY A.;KOSMEDER, JEROME W.;RODGERS, PAULA M.;WONG, JENNIFER |
分类号 |
C12Q1/68;C07F9/09;C07F9/655;C07H15/20;C07H15/24;C07H15/26;C12Q1/00;C12Q1/42;C40B30/04;G01N1/30;G01N33/53;G01N33/58 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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