发明名称 |
dsRNA for treating viral infection |
摘要 |
The invention relates to double-stranded ribonucleic acids (dsRNAs) targeting gene expression of phosphatidylinositol 4-kinase (PI4K), in particular human phosphatidylinositol 4-kinase, catalytic, beta polypeptide (PIK4CB) or human phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (PIK4CA), and their use for treating infection by positive stranded RNA viruses such as hepatitis C virus (HCV). Each dsRNA comprises an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of the PIK4CB or PIK4CA target mRNA. A plurality of such dsRNA may be employed to provide therapeutic benefit. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier, and including a delivery modality such as fully encapsulated liposomes or lipid complexes. The invention further includes methods for treating diseases caused by positive stranded RNA virus infection using the pharmaceutical compositions; and methods for inhibiting the propogation of positive stranded RNA viruses in and between cells. |
申请公布号 |
US9133462(B2) |
申请公布日期 |
2015.09.15 |
申请号 |
US201314069642 |
申请日期 |
2013.11.01 |
申请人 |
Arrowhead Research Corporation |
发明人 |
Labow Mark Aron;Gaither Larry Alexander;Borawski Jason |
分类号 |
C12N15/113;A61K31/713 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
1. A method of treating a pathological process mediated by positive stranded RNA virus infection comprising the step of administering to a patient in need of such treatment, prevention or management a therapeutically or prophylactically effective amount of a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of phosphatidylinositol 4-kinase (PI4K) in a cell, wherein said dsRNA comprises a first strand and a second strand, wherein the sequence of the first strand comprises the sequence of SEQ ID NO: 210. |
地址 |
Pasadena CA US |