Engineering microorganisms

摘要

The present disclosure provides, inter alia, Thraustochytrium and relevant methods and reagents, including engineered regulatory sequences from and/or operative in Thraustochytrid or Thraustochytrium, selectable markers useful for engineering microorganisms such as Thraustochytrids, methods for mutagenizing microorganisms, novel strains produced by mutagenesis and methods and compositions related to production of particular compounds in microorganisms.

主权项

1. A method for transforming a Thraustochytrium cell comprising the steps of:
(a) providing a competent Thraustochytrium cell; (b) delivering a recombinant nucleic acid into the competent Thraustochytrium cell, wherein the recombinant nucleic acid comprises a selectable marker and a tubulin promoter that is at least 80% identical to SEQ ID NO:10; and (c) culturing the competent Thraustochytrium cell in a culturing medium containing a selection agent that reduces growth of cells without the selectable marker.