System and method for sub-surface fluorescence imaging

摘要

A system for sub-surface fluorescence imaging is provided, the system comprising: an excitation source for selectably emitting light at at least one of at least two excitation wavelengths or wavelength ranges at a target surface; and a light detector for detecting fluorescence emission wavelengths or wavelength ranges from the target surface; wherein at least one of the at least two excitation wavelengths or wavelength ranges causes fluorescing of at least one marker at a sub-surface depth, the emitted light at each of the at least two excitation wavelengths or wavelength ranges having different depths of optical penetration and causing fluorescing at respective different depths. A method for sub-surface fluorescence imaging is also provided, in some cases exemplified by a reconstruction of the sub-surface fluorescence topography.

主权项

1. A system for sub-surface fluorescence topographical imaging comprising:
an excitation source for selectably emitting light at at least two excitation wavelengths or wavelength ranges at a target surface, the at least two excitation wavelengths or wavelength ranges comprising:
a first excitation wavelength or wavelength range selected to provide a first depth of optical penetration, for causing fluorescing of a marker below the target surface; anda second excitation wavelength or wavelength range selected to provide a second depth of optical penetration different from the first depth of optical penetration, for causing fluorescing of the marker below the target surface; and a light detector for detecting fluorescence emission wavelengths or wavelength ranges from the target surface.