| 摘要 |
A gene expression system is provided. The system comprises at least one coding sequence to be expressed in an organism, and at least one promoter operably linked thereto. It further comprises at least one splice control sequence which, in cooperation with a spliceosome, mediates alternative splicing of RNA transcripts of the coding sequence. The mediation of alternative splicing is in a sex-specific, stage-specific, germline-specific and tissue-specific manner. |
| 主权项 |
1. A gene expression system comprising a coding sequence to be expressed in an insect, a promoter operably linked thereto, and an intronic splice control sequence, which is flanked on its 5′ end by a guanine (G) nucleotide,
wherein said intronic splice control sequence, in cooperation with a spliceosome, is capable of sex-specifically mediating alternative splicing of RNA transcripts of the coding sequence, the coding sequence encodes a protein having a lethal, deleterious or sterilizing effect and is not a sequence associated with or linked to the intronic splice control sequence in a native context, wherein the intronic splice control sequence is obtained from AaActin-4, Dsx, Bztra or Cctra, and wherein the insect is selected from the group consisting of mosquito, bollworm and medfly. |
