Therapeutic RNA interference technology targeted to the PDX-1 oncogene in PDX-1 expressing neuroendocrine tumors

摘要

A bifunctional shRNA-based composition and methods for knocking down the expression of the PDX-1 oncogene in target cells is described herein. The invention also provides methods to deliver the shRNA-containing expression vectors to target tissues overexpressing the PDX-1 oncogene.

主权项

1. An expression vector comprising:
a promoter; and a nucleic acid insert operably linked to the promoter, wherein the insert encodes one or more short hairpin RNAs (shRNA) capable of hybridizing to a region of an mRNA transcript that encodes a PDX-1 oncogene and that inhibits the PDX-1 oncogene expression via RNA interference, wherein the insert comprises both an siRNA (cleavage-dependent) and miRNA (cleavage-independent) motif, wherein the one or more shRNA comprise a sequence selected from the group consisting of SEQ ID NOS: 3-8, and combinations or modifications thereof.