METHOD FOR ASSESSING THE VIABILITY OF VIRUSES WITH LYMPHOTROPISM

摘要

Use: medicine and biotechnology. Aim: to increase the reliability of determining infection by viruses with lymphotropism to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during EIA and PCR testing and to detect viruses with lymphotropism in biological material having a concentration of virus particles lower than the sensitivity threshold of EIA or PCR methods. Essence of the invention: the method for assessing the viability of viruses with lymphotropism comprises collecting biological material and determining whether said material contains virus RNA or DNA by means of conducting a polymerase chain reaction (PCR reaction). In addition a lymphocyte suspension is taken from the blood of healthy people to which lymphocytes an equal volume of biological material is added. This combination is then mixed incubated at a temperature of 37°? for a period of 6 8 hours and the lymphocytes are washed of plasma and broken down. The lymphocyte cytoplasm is then subjected to PCR testing. The detection of virus RNA or DNA in the lymphocyte cytoplasm indicates that the viruses have retained their viability. The absence of virus RNA or DNA in the lymphocyte cytoplasm indicates the inactivation of the viruses.