ELECTRONIC METHODS AND SYSTEMS FOR MICROORGANISM CHARACTERIZATION

摘要

Systems and methods to characterize one or more microorganisms or DNA fragments thereof are disclosed. Exemplary methods and systems use comparison of DNA sequencing information to information in one or more databases to characterize the one or more microorganism or DNA fragments thereof. Exemplary systems and methods can be used in a clinical setting to provide rapid analysis of microorganisms that may be a cause of infection.

主权项

1. A method of characterizing one or more microorganisms, the method comprising the steps of:
selecting, by a computer, a digital file comprising one or more digital DNA sequences, wherein each of the one or more digital DNA sequences corresponds to a microorganism to be characterized; segmenting, by the computer, each of the one or more digital DNA sequences into one or more first portions; performing, by the computer, a set of alignments by comparing the one or more first portions to information stored in a first database; determining, by the computer, sequence portions from among the one or more first portions that have an alignment match to the information stored in the first database; performing, by the computer, a set of alignments by comparing the one or more first portions or the one or more second portions to information stored in a second database; determining, by the computer, sequence portions from among the one or more first portions or one or more second portions, wherein the second portions are formed by segmenting, by the computer, each of the one or more digital DNA sequences into one or more second portions, that have an alignment match to the information stored in the second database; and characterizing one or more microorganisms or DNA fragments thereof based on the alignment match to the information stored in one or more of the first database and the second database.