PURIFIED PREPARATION OF URATE OXIDASE, PURIFIED RECOMBINANT URATE OXIDASE, CONJUGATE (VERSIONS) AND PHARMACEUTICAL COMPOSITION FOR REDUCING MAMMALIAN LIQUID OR TISSUE URIC ACID, PURIFIED FRAGMENTS OF URATE OXIDASE AND METHOD FOR URATE OXIDASE PURIFICATION

摘要

FIELD: medicine, pharmaceutics.SUBSTANCE: present invention refers to biotechnology and represents a purified recombinant urate oxidase (uricase) characterised by the tetramer and octamer content of 95% and more of the total molecule count. The invention also discloses a purified preparation of the above urate oxidase possessing lower immunogenicity, a conjugate of the above urate oxidase and polyethylene glycol or polyethylene oxide used to reduce mammalian tissue or liquid uric acid, purified fragments of the above urate oxidase, a pharmaceutical composition containing the above urate oxidase and a method for purifying the above urate oxidase with maintaining its uricolytic activity, involving the separation and removal of uricase aggregates bigger than octamers.EFFECT: present invention enables producing the preparations of urate oxidase of lower immunogenicity.23 cl, 6 dwg, 6 ex