摘要 |
The invention relates to a method for producing a glycoconjugate comprising an oligosaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, using a genetically modified cell. |
主权项 |
1. A method for producing a glycoconjugate comprising an oligosaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, the method comprising:
(i) providing a genetically modified cell comprising a gene encoding a glycosyl transferase enzyme that is able to transfer the glycosyl residue of an activated sugar nucleotide to an internalized acceptor, (ii) culturing said genetically modified cell in the presence of an exogenous acceptor comprising a mono- or disaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, inducing
(a) internalization of the exogenous acceptor by the genetically modified cell, and(b) formation of said glycoconjugate from said internalized acceptor molecule by a glycosyl transfer mediated by said glycosyl transferase enzyme expressed by said cell, and then (iii) collecting said glycoconjugate from the fermentation broth. |