BIOLOGICAL CELL SEPARATOR AND DISPOSABLE KIT

摘要

A disposable kit for use in directing fluid through a biological cell separator device (10). The kit generally includes a separator tube (22), a buffer fluid container (34), cell sample container (32), separated cell container (60), and flushing fluid container (62), as well as various conduits (36, 38, 42, 50, 50a, 50b) for connecting the containers (32, 34, 60, 62) and separator tube (22) in fluid communication together. A cell separator system is provided including a separator tube (22), magnet (20), pump (120) and a motorized drive unit (96). The motorized drive unit (96) is operatively connected to the magnet (20) to allow the magnet (20) to be moved a sufficient distance away from the separator tube (22) so as to allow cells adhered to the inside surface thereof to be flushed out of the tube (22).

主权项

1. A disposable kit for directing a fluid sample of biological cells through a separator device and collecting separated cells from the sample, the kit comprising:
a sample receiving container including an inlet and an outlet, the inlet coupled with an inlet conduit for receiving a buffer fluid and the outlet coupled with an outlet conduit, the outlet conduit comprising a first section and a second section; a separator tube including an inlet end and an outlet end, the inlet end coupled with the outlet conduit from the sample receiving container, the separator tube having:
a length defining a separation channel;a diameter adapted for operable communication with a magnet along the length of the separation channel;a space for receiving a flow of the buffer fluid and sample, and wherein a length of the second section of the outlet conduit from the sample receiving container is oriented along a path defined by the separator tube and adapted to allow displacement of the magnet from a separation position to an idle position while the flow of fluid is maintained;a separated cell container having an inlet port and a vacuum port,a separated cell conduit coupled for fluid communication between the outlet end of the separator tube and the inlet port of the separated cell container,a flushing fluid container having an inlet port and a vacuum port; anda flushing conduit coupled for fluid communication between the outlet end of the separator tube and the inlet port of the flushing fluid container.