Method for Production of Single-Stranded Macronucleotides

摘要

The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.

主权项

1. A method for production of single-stranded nucleic acids, comprising the steps:
a. provision of a desired single-stranded target nucleic acid sequence (targetss), which contains at least one monomer of a desired nucleic acid sequence, b. extension of the desired nucleic acid sequence by additional functional or non-functional nucleic acid sequences at one or both flanking ends to obtain an extended single-stranded target nucleic acid sequence (targetss), c. amplification of the extended monomeric single-stranded target nucleic acid sequence (targetss) into a double-stranded target nucleic acid sequence (targetss), d. ligation of the double-stranded target nucleic acid sequence (targetds) to produce a repetitive cluster of the double-stranded target nucleic acid sequence (aptagene), e. cloning of the aptagene produced in step d. into a vector (aptagene vector), f. transformation of the aptagene vector into host cells for replication of the aptagene, g. isolation of the aptagene from the host cells and division of the isolated aptagene to multiple copies of the double-stranded target sequence (targetds), h. degradation of the double-stranded target sequence (targetds) to single-stranded target sequences (targetss).