METHODS AND KITS USEFUL IN THE DIFFERENTIATION OF BURKHOLDERIA SPECIES

摘要

The invention pertains to sequences, methods, and kits useful in the identification of and differentiation between Burkholderia pseudomallei and Burkholderia mallei. The methods generally involve the addition of oligonucleotides to mixtures containing nucleic acid isolated from a sample and performing nucleic acid amplification on the mixture.

主权项

1. A method of determining the presence or absence of B. mallei in a sample, comprising the steps of:
receiving a nucleic acid-containing sample from a subject; adding a first oligonucleotide to a mixture comprising said nucleic acid, said first oligonucleotide including a sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, and SEQ ID NO. 3; subjecting the mixture to conditions that allow amplification of said nucleic acid; and identifying said sample as containing B. mallei or not containing B. mallei on the basis of a result of the nucleic acid amplification.