QUANTIFICATION OF TAU IN BIOLOGICAL SAMPLES BY IMMUNOAFFINITY ENRICHMENT AND MASS SPECTROMETRY

摘要

The present invention provides a quantitative immunoaffinity LC-MS/MS assay for detection and quantification of Tau protein in a biological sample.

主权项

1. A method for measuring Tau protein in a biological sample comprising the steps of:
a. providing a biological sample suspected of comprising Tau protein; b. adding an isotope labeled internal standard Tau protein to the sample to produce a spiked sample; c. contacting the spiked sample with an anti-Tau antibody that is covalently conjugated to solid phase particles; d. maintaining the sample produced in step c) under conditions suitable to allow the anti-Tau antibody to bind to Tau protein present in the sample; e. washing the particles; f. eluting the Tau protein from the particles; g. contacting the protein recovered in step e) with trypsin under conditions suitable to digest the Tau protein into an analyte sample comprising a composition of peptides, and; h. performing mass spectroscopic analysis to detect and measure the concentration of a surrogate Tau peptide present in the analyte sample.