Peptide Analogues of PA-IL and Their Utility for Glycan and Glycoconjugate Analysis and Purification

摘要

Provided are peptide analogues of PA-IL and compositions containing them. The PA-IL peptide analogues have altered carbohydrate binding specificity relative to a PA-IL of SEQ ID NO:1, and thus the analogues contain amino acid substitutions in SEQ ID NO:1. The substitutions can be at positions 50, 52 and 53 of SEQ ID NO:1 and can include combinations of amino acid substitutions at those positions. Also included are methods for detecting changes in the glycosylation of carbohydrates and for separating biomolecules which contain glycoproteins or glycoconjugates.

主权项

1. A peptide analogue of PA-IL of SEQ ID NO: 1 comprising an amino acid substitution at one, two or three of positions 50, 52 and 53;
wherein the amino acid substitution at position 50 is selected from the group consisting of Ala, Val, Leu, Ile, Met, Phe, Trp, Pro, Ser, Thr, Cys, Tyr, Gly, Asn, Asp, Gln, Glu, Lys, and Arg; wherein the amino acid substitution at position 52 is selected from the group consisting of Ser, Thr, Cys, Asn, Gln, Asp, Glu, Lys, Arg and His; and wherein the amino acid substitution at position 53 is selected from the group consisting of Ala, Val, Leu, Ile, Met, Phe, Trp, Pro, Gly, Ser, Thr, Cys, Tyr, Asn, Asp, Glu, Lys, Arg and His.